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L2766
Sigma
Lectin from Datura stramonium (jimson weed, thorn apple)
lyophilized powder
| Synonym: | Datura stramonium lectin, DSL |
| MDL number: | MFCD00163424 |
Description
| Analysis Note | Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution in phosphate buffered saline, pH 6.8. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C. |
| Biochem/physiol Actions | DSL is not blood group specific but has an affinity for oligomers of N-acetyl-glucosamine and N-acetyllactosamine. The lectin is a glycoprotein containing approx. 35% carbohydrate. |
| Preparation Note | Purified by affinity chromatography |
| Quality | Highly purified. |
Properties
| form | lyophilized powder |
| potency | ≤10 μg per mL agglutination activity |
| composition | Protein, ≥60% biuret |
| total impurities | salt, essentially free |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Crowley, J.I., et al. Arch. Biochem. Biophys. 231, 524, (1984) |
| Young, N.M., et al. Biochem. J. 222, 451, (1984) |
